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Description

Quantitative analysis of the thioflavin-S and GSK-3 positive area was performed (ImageJ ) and interfaced with a digital CCD camera mounted on a fluorescent microscope (TissueGnostics, Austria)

After day 1, the ileum organoids were maintained in expansion medium supplemented with either 1 mM Mn 2+ or 5 nM scTS2/16, with the medium refreshed every 23 days

They designed the assays independently, analyzed the reference samples with their assays of choice and submitted the final results together with a detailed assay report for centralized benchmarking analysis by the study coordinator