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Ci et al

The same crude extract was utilized in all the bioassays described herein

[nature] 23

Tumor cells were immersed in RBC lysate to remove erythrocytes, washed and centrifuged several times, then dispersed in PBS and stained with CD8 (Elabscience, E-AB-F1104E, Clone: 53-6.7), CD4 (Elabscience, E-AB-F1353C, Clone: RM4-5) and CD3 (Elabscience, E-AB-F1013D, Clone: 17A2) for flow cytometry analysis