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10.1016/j.diagmicrobio.2017.09.007 66

Immunofluorescence staining of brain tissues For brain tissue immunostaining, mice were transcardially perfused with cold PBS followed by chilled 4% PFA in 0.1 M phosphate buffer (pH 7.2), and brains were subsequently paraffin-embedded

Furthermore, dihydrobiopterin plays a role in maintaining the reduced state of tetrahydrofolate, so the clinical phenotype of dihydrobiopterin deficiency is similar to that of dihydrofolate reductase deficiency

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