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Landmark studies on the origin of (excessive) thalamo-cortical burst firing revealed that the balance between inhibition and excitation in thalamo-cortical networks is effective in setting the firing pattern of thalamo-cortical relay neurons by controlling the activation of low-threshold voltage-gated Ca 2+ channels (as reviewed in ref [103])

Using RNase-free water when working with RNA prevents degradation and preserves the quality of the samples

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Decrease dose consideration: Persistent side effects including headaches, fatigue, or injection site reactions, excessive response (extremely rapid weight loss suggesting dose may be higher than needed), cost concerns when lower doses might achieve similar results