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However, considerable clinical problems on bone defect are so far unaddressed, with a remaining group of 10% to 20% of delayed or nonunion situations [2]

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The success of using introduced cysteine residues for site-specific conjugation relies on the ability to select proper sites in which cysteine-substitution does not alter protein structure or function

Autophagy is a crucial act of intracellular self-digestion, capable of transporting proteins and organelles from the cytoplasm to the lysosome for degradation (Mizushima et al., 2008)