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Zhonghua Fu Chan Ke Za Zhi 53 (12), 836841

In addition to hereditary hemoglobinopathies, aberrant Gardos channel activity has been associated with storage lesions, wherein increased intracellular Ca 2+ concentrations progressively activate the channel, leading to cellular shrinkage and diminished deformability under extended refrigeration (11, 36)

The binding reaction was performed in a 20 l reaction mixtures containing 8 g of nuclear extracts from NA cells, 1 M Biotin-labeled probes, 20 mM Tris-HCl, 50 mM KCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM dithiothreitol, 10% glycerol, and 2 g of poly (dI-dC)

Others were reported to have occurred hours to days after use concurrent with sexual activity