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The SDS-PAGE gel was transferred onto nitrocellulose membrane (Thermo Scientific) that was then treated with 100 mM sodium borohydride (Fisher Chemical) for 20 minutes to stabilize 4HNE adducts (McCormack et al., 2005)

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(Heres the lab work you actually need.) What does follow-up look like

166,190 Further, in gastrointestinal cancer cells, genetic inhibition or pharmacological treatment of CPT2 with perhexiline disrupts NADPH and promotes cell apoptosis after oxaliplatin treatment