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the present inventors recognize a solution to prevent in vivo target protein cleavage using split-inteins whose catalytic residues are split between two peptide chains: the N-terminal intein (IN) and the C-terminal intein (IC)

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Suv ML, Rheinbay E, Gillespie SM, Patel AP, Wakimoto H, Rabkin SD, et al

In most cases, the intein self-cleavage reaction is essentially complete in 4 hours, keeping the time to complete the entire procedure on par with conventional chromatographic protein purification processes