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Synthesised cDNA or control reaction (0.51L) were added to separate PCR mixtures containing final concentrations of 5L PowerUpTM SYBR Green PCR Master Mix (Applied BiosystemsTM, Waltham, Massachusetts, USA) and 3L RNase-free water to a final volume reaction mixture of 10L and 2 M sense and antisense primers 15,16 ( Table 1 )

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