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Enzyme-linked immunosorbent assay kits were used to determine the levels of pro-inflammatory cytokines, specifically TNF- and IL-6, in cell culture supernatants

For quality control, the standards and a random sample were re-measured for every batch of 20 samples with relative standard deviations (RSDs) 1.28-fold and p 0.9)

Astroglia respond to external, non-cell autonomous signals associated with any form of CNS pathology by undergoing complex and variable changes in their structure, molecular expression, and function

Here we searched for examples of protein substrates where the number of redox-active cysteine residues has changed throughout evolution