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Focal adhesion dynamics: Cell migration requires coordinating adhesion formation at leading edge and adhesion dissolution at trailing edge

CDC Calcein-AM release assay Raji, WSU-NHL, DOHH2, SU-DHL-4 cells were washed in 10 mL HBSS and resuspended to 1.0 10 7 cells/mL in HBSS before stained with 1 M Calcein-AM (Merck) for 20 min at RT