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These conserved Cys residues may serve as ligands for Fe-S clusters

Thus, IGF-1 signaling on Th17 cells may have evolved to protect the Th17 response from potential damage due to high metabolic flux

The compounds used are Ferrostain-1 (Fer-1) (S7243, Selleck), Propidium iodide (PI) (P4170, Sigma), BODIPY581/591 C11 (D3861, ThermoFisher), DCFH2-DA (D6883, Sigma), Cystine (30200, Sigma), Methionine (Met) (M9625, Sigma), RhoNox-1 (HY-D1533, MCE), -Vitamin E ((+)--Tocopherol) (HY-N0683, MCE), RSL3 (HY-100218A, MCE), DL-Propargylglycine (HY-W040124, MCE), Selenomethionine (HY-B1000, MCE), L-Selenocystine (HY-129960, MCE), Cisplatin (HY-17394, MCE), Cycloheximide (HY-12320, MCE), FDA-Approved Drug Library (HY-L022, MCE), Natural Product Library (HY-L021, MCE), Dulbeccos Modified Eagles Medium high glucose without L-methionine, L-cystine, and L-glutamine (D0422-100 ml, Sigma), and Penicillin-Streptomycin (P06-07100, Pan Biotech)

doi: 10.3390/cancers17050817 92 ElguindyMYoungJSMondalILuROHoWS