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For histological analysis, the femoral and tibial condyles were fixed in 10% neutral buffered formalin, decalcified with 20% EDTA, embedded in paraffin, and sectioned into 5-m thick frontal slices

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After amplification, PCR products were electrophoresed on agarose gels and transferred onto nylon membranes using Southern blotting techniques

If a patient has one of more of these high-risk features, it is very important that the disease is managed by an experienced gastroenterologist with expertise that includes IBD