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All of the cells were incubated at 37 C in an incubator with 5% CO 2 and a humidified atmosphere

2.5 l of the sample was subjected to pre-column derivatization with OPA reagent (Agilent, 50613335) by programming the auto-sampler and the separation of amino acids was carried out in Agilent 1260 Infinity HPLC System (Agilent Technologies) using a Poroshell 120 HPH-C18 column (4.6 mm 100 mm X 2.7 m) as per the manufacturers protocol 70

Watkin K

Xu R, Lou Y, Tidu A, Bulet P, Heinekamp T, Martin F, Brakhage A, Li Z, Ligeois S, Ferrandon D (2023) The toll pathway mediates Drosophila resilience to Aspergillus Mycotoxins through specific bomanins