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Dose dependent differences in orexin A effects between our results and previous study may be related to a lower peptide concentrations used in our experiment

The Cterminal motif from human 4ET spanning residues 954985 (4ET C ) was expressed fused to an Nterminal glutathione Stransferase (GST) affinity tag followed by the human rhinovirus 3C protease cleavage site

Liu J, Chen Y, Shan X, Wang H

Although it might seem relevant to determine substrate specificity by assaying the oxidoreductase activity of individual PDI family members using purified enzymes and substrates, it should be understood that within cells it is unlikely that these enzymes function in isolation