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After additional three 10 min washings in TBS with 0.3% (v/v) Tween 20, protein signals were revealed using the Chemiluminescence (ECL) detection system (EuroClone, Pero, Milan, Italy)

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It classically produces a loud S1, an opening snap, and a mid-to-late low-pitched diastolic rumble heard best at the cardiac apex

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556 Interestingly, the expression of SIRT1 in the spinal cord of WT mouse is reduced during normal aging, while the expression of SIRT1 in different regions of the brain (including the spinal cord, hippocampus, thalamus and cerebral cortex) in SOD1G93A mouse is increased