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Functional impairment of coronary microvascular function may be triggered by low-grade systemic inflammation [19, 20], reduction of nitric oxide (NO) bioavailability, increased levels of vasoconstriction mediators [21], increased oxidative stress, increased leukocyte infiltration, neurohormonal activation, microvascular wall barrier dysfunction and altered vascular endothelial permeability [22]

The following day, Ni-NTA beads were collected through centrifugation at a low speed (Eppendorf 5810 R, Rotor 1730 g for 10 min) and collected into one 50 mL Falcon tube and washed twice with 50 mL of buffer A

Invert the vial and draw the bacteriostatic water slowly, ensuring no air bubbles enter the syringe

Each serving provides 25 grams of protein from higher-quality whey protein isolate