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In addition, human adipose-derived stem cells maintained viability following exposure to low-dose CAP-activated solution without losing phenotypic properties, defining a translational window of safety and efficacy for combined CAP-based and biologic or material-assisted approaches in subcutaneous tissues that can be used for contour maintenance [283, 284]

Currently, this hypothesis is replaced by the multiple hit hypothesis that considers multiple factors (such as lipotoxicity, diet, sedentary lifestyle, epigenetics, insulin resistance, innate immune activation, environmental pollution, etc

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PhoA accumulation was analyzed by SDS-PAGE (NuPAGE 4 bis 12 %, Bis-Tris, Invitrogen, Waltham, MA, USA) and Western blot analyses were carried out using nitrocellulose membranes in the iBLOT2 system (Invitrogen, Waltham, MA, USA), both according to the supplier