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The most effective approach is to gently explore his internal motivations using open-ended questions, making the most appropriate physician statement "Are there any reasons why you might want to quit smoking?" In the precontemplation stage , patients do not intend to take action in the foreseeable future and may be unaware that their behavior is problematic

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In the oxidized state with two nearby cysteines forming a disulfide bond and the third cysteine unmodified, HMGB1 acts as a proinflammatory cytokine (Yang et al., 2021) which can interact with TLR4 (Pauletti et al., 2019)