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Moreover, the discovery of IRAP inhibitors by virtual and substance library screening and direct design applying knowledge of the structure of IRAP and of related enzymes is briefly presented

The levels of samples AGEs (Sigma-Aldrich, MO, USA), TNF- (Sigma-Aldrich, MO, USA), IL-6 (Sigma-Aldrich, MO, USA) and collagen-I (Sigma-Aldrich, MO, USA) were quantified using the enzyme linked immunosorbent assay (ELISA) detection kit according to the manufacturers instructions