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Furthermore, esculetin (5 mg/kg) can inhibit the production of NO, TNF-, IL-6, iNOS and nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing in DSS-induced colitis rats by inhibiting the activation of the NF-B and MAPK signaling pathways (86,87)

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The supernatant was centrifuged again at 10,000 rcf for 5 min at 4 C