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For comparing multiple data sets, a one-way ANOVA with multiple comparisons with Tukey post-hoc test was used for normal distributions, and a Mann-Whitney U test was used for non-normal distributions

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Moreover, calcium mobility assays using synthesized ghrelin with fatty acids of various lengths revealed that ghrelin is less active when modified with a shorter-chain fatty acid 12

Interestingly, in this case, although there were no particularly noticeable changes in blood pressure or heart rate , there was a marked increase in sweating during the central autonomic storm, This dissociation of autonomic symptoms suggests that there may be a functional localization within the central autonomic nervous network