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Following transient and stable expression in Nicotiana and Arabidopsis, respectively, the GSTs were recovered using Strep-Tactin affinity chromatography and the bound ligands desorbed and characterized by LC-MS

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however, some xenobiotics, ionizing radiation, illnesses, or aging may cause the production of ROS to levels that exceed the neutralizing capacity of an organism, thus leading to a series of pathological changes, which in turn leads to oxidative stress