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Then 200 nM (200 l) of NIP-specific IgG1 variants were added to either the apical or basolateral reservoir and incubated at 37 C for 4 h before samples were taken from the opposite reservoir and analyzed by ELISA

To address organelle-specific pH and redox environments, turn-on zinc fluorescent probes (ZnDA-2H and ZnDA-3H) with low pH sensitivity and high affinity were developed and targeted to the cytosol, nucleus, ER and mitochondria using HaloTag technology, enabling the precise quantification of labile zinc distribution 189

Concurrently, lipid biosynthesis is enhanced, while FAO is suppressed, supporting cell membrane expansion and promoting signal transduction

PMC 3999603