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If your protocol involves a single administration or requires the entire vial to be used within three days, sterile water is acceptable, but for multi-dose protocols, bacteriostatic water is non-negotiable

Inhibition of tyrosinase (the key enzyme of melanogenesis): Direct inhibition: Thiol group binding with the copper-containing active site of the enzyme Indirect inactivation: Exerted via the antioxidant effect of glutathione that leads to quenching of free radicals and peroxides Switching production of eumelanin to phaeomelanin Modulation of the depigmenting properties of other antimelanogenic principles Table No * N * F * 100] / W Where VU = Titrant volume of sample (ml) Vb = Titrant volume of blank (ml) N = Titrant Normality F = Equivalence Factor W = Weight of Sample (mg) Acceptance Criteria: 98.0% - 101% on the dried basis

doi: 10.1002/jcph.1008 156 SmeyneMSmeyneRJ

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