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Each reaction contained 100 ng of kinases and 500 M, 250 M, 50 M or 25 M of biotinylated substrate peptide (Anaspec)

All individual constructs designed for this study are schematically represented in Supplementary Fig

The most abundant ones in grape are called proanthocyanidins, and 60-70% of them are found in the seeds (it's also often abbreviated as GSP - grape seed proanthocyanidins)