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The sepharose beads were then washed three times with lysis buffer, and boiling in 50 L 2 SDS loading buffer

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Additionally, administration of live Escherichia coli Nissle 1917 (EcN) only before or during disease induction significantly reduced clinical scores in EAU, indicating that the immunoregulatory effects of EcN preceded the development of EAU and influenced the initial antigen presentation and T cell (IRBP-specific CD4+ T cell) activation in the draining lymph nodes of immune sites (115)