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This limitation arises from the use of the ESI-MS/MS technique based on the in-solution buffer-free digestion method employed in this study to characterize RBD variants, which is not currently quantitative for such analysis ( In short, S-alkylation of C538 in the RBD protein leads to multiple chemical modifications resulting from free cysteines being exposed

The physiological regulation and cross-talk among individual hydrolases remains largely uncharted investigative territory and is likely to reveal important new roles in signalling and neuronal dysfunctions more subtle than degeneration 239,240