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The Ang IV analogue peptide 4 and the Nle-Ang IV analogue 5 provide examples comprising two basic amino groups accessible for protonation in the N-terminal of the peptides and flanking hydrophobic 2-isopropyl and n-butyl side chains, respectively (Figure 2) (Sardinia et al., 1994)

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These compounds appear in the published neuropeptide-signalling literature, much of it originating from Russian research groups, in pre-clinical, in-vitro and animal model systems